High-speed FLIM with SPAD arrays

Traditionally, Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Resonance Energy Transfer (FRET) imaging have used scanning systems to form an image of the sample being tested.

  • Photon Force’s PF32 Time-Correlated Single-Photon Counting (TCSPC) SPAD array cameras can be used to create an image with no need for scanning. In fact, users can acquire video rate FLIM in the time previously taken to collect a single static image.

Fluorescence Lifetime Imaging microscopy

See Photon Force assisted FLIM imaging in action

  • The video below is an excerpt from the paper ‘A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging’ by Simon P. Poland et al, where the PF32 SPAD array camera was used in conjunction with a two dimensional array of ultrafast beams – the multibeam confocal architecture. This parallelism of excitation and detection allowed FLIM FRET imaging to be performed at unprecedented speeds.

  • The image on the left of the video shows intensity, the middle image depicts lifetime and the right-hand image combines both intensity and lifetime.

Time-resolved measurements for FLIM imaging

Photon Force technologies could make a significant impact on your FLIM project. Want to know more?